Journal: JOR Spine

Article Title: Age‐related molecular changes in the lumbar dorsal root ganglia of mice: Signs of sensitization, and inflammatory response

doi: 10.1002/jsp2.1124

Figure Lengend Snippet: List of primary and secondary antibodies used for immunofluorescence analysis

Article Snippet: rabbit anti‐NGF , BosterBio , m00341 , 1.00 , 1:100 , same as above.

Techniques: Immunofluorescence, Concentration Assay

Journal: Translational Vision Science & Technology

Article Title: Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines

doi: 10.1167/tvst.10.6.27

Figure Lengend Snippet: Xeno- and Feeder-Free Differentiation by Recombinant Cytokines

Article Snippet: Human iPSCs were then cultured in stage 1 differentiation medium containing MEM-α, 10% KnockOut Serum Replacement (Thermo Fisher Scientific) and 2 ng/mL TGF-β 1 , 100 ng/mL NGF-β, and 2 U/mL EPO (Sino Biological, Beijing, China) for 7 days.

Techniques: Recombinant, Concentration Assay, Knock-Out

Journal: Translational Vision Science & Technology

Article Title: Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines

doi: 10.1167/tvst.10.6.27

Figure Lengend Snippet: Xeno- and feeder-free differentiation with recombinant cytokines. ( A ) Diagram of the new differentiation approach with two distinct stages. The first stage is to induce iPSCs to differentiate for 7 days by using TGF-β1, NGF-β, and EPO. Two more recombinant cytokines were involved in the differentiation of the second stage (14 days), including PGF2α and EGF. ( B ) ELISA analysis of the cytokines in the medium conditioned by HTM cells. Observable levels of TGF-β 1 , NGF-β, EPO, EGF, and PGF2α, as the ligands of the selected receptors, were found in the conditioned medium of HTM cells ( n = 3). The experiment was repeated three times by using HTM cells of three donors.

Article Snippet: Human iPSCs were then cultured in stage 1 differentiation medium containing MEM-α, 10% KnockOut Serum Replacement (Thermo Fisher Scientific) and 2 ng/mL TGF-β 1 , 100 ng/mL NGF-β, and 2 U/mL EPO (Sino Biological, Beijing, China) for 7 days.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: Investigative Ophthalmology & Visual Science

Article Title: Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion

doi: 10.1167/iovs.63.8.3

Figure Lengend Snippet: Antibodies Used in Microarray Analysis

Article Snippet: Anti-nerve growth factor beta ( NGFb ) , ORB11126, Biorybt, Cambridge, United Kingdom.

Techniques: Microarray